Biochemistry 2280A Lecture Notes - Lecture 34: Alanine, Stop Codon, Nucleosome
12 Dec 2016
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Topic 28: why sequence genomes? parts of the puzzle ) Identifies all the genes that characterize an organism ( identifies all the. Identifies potential drug targets (example/ parasites that have unique genes: sequencing a genome involves: Aligning the independent sequences into a continuous sequences: genomic library. A collection of cloned dna fragments that represent all of the dna in an organism"s genome. Each cloned dna is like a different book: constructing a genomic library. You have lots of human dna isolated from many cells restrict or cleave it with restriction enzyme (endonuclease) this gives you a whole bunch of. Dna fragments with the fragments, you clone them into a plasmid by ligation reaction transform them into bacteria and get a whole bunch of clones = genomic library (figure 10-11) Each of these clones have a different fragment of dna and together they represent the whole genome.
