Biochemistry 2280A Lecture Notes - Lecture 21: Polymerase Chain Reaction, Hybridization Probe, Recombinant Dna
20 Feb 2017
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Topic 21 & 22: recombinant dna technology genetic engineering. Definition: the techniques which recombine dna fragments from different sources (different chromosomes, different organisms, or man-made) into new molecules with unique features. Clicker question: in a dna sequencing reaction you generally add ~100 ng of dna. Assuming the dna is 3000bp and one base pair has a mass of ~600 daltons, approximately how many molecules are added: 30, 3 x 105, 3 x 1010, 3 x 1020. 100 x 10-9 g / 1800 000g/mol = 5. 55 x 10-14 mol = 3. 34 x 1010. Synthetic biology engineering of biological systems, with the goal of carrying out novel functions or performing existing functions more efficiently. People have manipulated genes for thousands of years using classical genetic selection. Plant/crop and livestock breeding are age-old examples of humans manipulating the dna of other organisms. Limited by breeding time of organisms and chance genetic events. Rapid: as quick as a few days in some organisms.
