Biochemistry 2280A Lecture Notes - Lecture 11: Flavoprotein, Atp Hydrolysis, Ribose

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Cofactor: protein-bound non- protein chemical, non-chemical chemical helps protein do its job. Not the same as enzyme where enzyme is regenerated. Coenzyme can be a substrate for a reaction eg. coa. In citric acid cycle carbons from glucose are oxidized to co2 and reduce nad+ and fad to nadh and fdh2. As electrons are transferred from complex to complex in etc it results in a loss of free energy which can be used to move h+ Each complex contains an enzyme the catalyzes electron transfer and several cofactors that accept/ donate electrons, the cofactors are tightly bound to the protein, thus are prosthetic group. Complexes are arranged in increasing redox potential in which complexes increase in affinity for electrons. Redox potential refers to the affinity of electrons, the more positive the redox potential the greater the affinity for electrons. Carriers electron affinity is dependent on its chemical structure and the enviroment.

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