Biochemistry 2280A Lecture Notes - Lecture 15: Dna Replication, Semiconservative Replication, Proofreading
25 Nov 2018
School
Department
Course
Professor
Document Summary
Initiation: priming, synthesis, proofreading, ligation, replication fork and replication bubble, telomeres, replication inhibitors. Overview of dna replication: replication is semi-conservative. In the new double helix, half of the. Dna has been conserved from the original strand. The other half is new: semi-conservative nature of dna replication was discovered by meselson and stahl in 1958. Initiation: replication doesn"t start randomly on dna. Specific locations called replication origins is where replication will start. In bacteria, it"s easy to see where the origin is based on the known replication origins. In bacteria, replication starts in one place only. Whole genome gets replicated in that one place only; goes around the circle. In humans, we have a much more complicated genome much bigger and divided into multiple linear chromosomes: we need at least one replication origin on each chromosome, but that"s not even enough. In fact, eukaryotes have many thousands of replication origins (30-
