Biochemistry 2280A Lecture Notes - Lecture 21: Dna Ligase, Aquabounty Technologies, Ethidium Bromide
Modern recombinant dna technology: genetic engineering, or recombinant dna technology, have led to discovery of new classes of genes, rnas, and proteins, has influence on treatment of disease, recombinant dna technology can play a role in fixing: Impacts on forensic science, laundry detergents, and more: global warming, food crisis, water crisis, global pandemic (ebola, sars, avian flu, antibiotic resistant bacteria. The exchange of genetic materials is also limited to breeding species: recombinant dna technology are extremely rapid as quick as a few days in some organisms. There are no limitations in terms of species. Isolating and manipulating genes is hard unlike proteins, genes are not a discrete entity in cells. 8 bp target = 1 in 65,536 (4^8: once dna molecules are cleaved into proper size pieces, gel electrophoresis separates. Expressing yfp in e. coli: first issue we need to think about is we need a source of the gene.