Biochemistry 2280A Lecture Notes - Lecture 30: Rna Virus, Variable Number Tandem Repeat, Chromosome

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Uses of pcr: rapid isolation of yfg, this was the basis of our case study, can produce cdna (from mrna) or genomic dna c. Fig 10. 17: analysis of bacteria or viruses in clinical or environmental samples, clinical - you want to test somebody to see if they are hiv positive. Get a small blood sample, isolate the dna. Since hiv is a rna virus, you have to reverse transcribe it. Then you use pcr to amplify it using primers for the hiv genome. If the hiv genome is there, we will see this pcr product on the gel: environmental - you want to know if your water sample is contaminated with e. coli . Take a water sample, extract nucleic acid from it and pcr for a e. coli gene using a specific primer: because of its sensitivity, it is very powerful one copy of the genome is enough, fig 10. 18.

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