Biochemistry 3386B Lecture 8: Lecture 8 - Urinanalysis.docx
15 Jul 2014
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Objectives: proper methods for urine specimen collection and handling: Urine can be collected randomly (single collection morning) or timed (several collected over time) If microscopic examination is to be done it must be within 2 hours of collection so that leukocytes or renal casts don"t disintegrate, and recycled after 4 hours (or microculture growth or metabolite pollution may occur ) Cloudiness may occur in fridge (nitrate and phosphate precipitation), persistent cloudiness may be bacterial growth. Ketone smell diabetes red colour candies/food/medication but usually blood, Refrigerated urine may be dipsticked post 2 hrs but no microscopic examination can be done. Urine is tested at room temp: principles of dipstick tests and their chemical reactions: Adjustable to accomade densities, color intensities and acidities and provides constant timing between measurements. Bilirubin normal negative always conjugated bilirubin. Unusual colors may suggest bile pigment presence that mask bilirubin reaction.
