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Lecture 5

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Biology 1001A
Tom Haffie

Independent Study Outcomes 1. purine and pyrimidine base-pairing in DNA/RNA 2. outcome of the classic Meselson and Stahl experiment 3. direction of movement of DNA polymerase on the template strand 4. meaning of semi-conservative, semi-discontinuous, leading and lagging strand 5. general action of proteins in Fig. 12.15. 1. purine = nitrogenous bases built from fused C and N rings adenine and guanine pyrimidine = built from single carbon ring thymine and cytosine 2. -predict DNA banding patterns and results support semi- conservative model of DNA replication 3. template strand uses dna polymerase at five prime to three prime 4. semi-conservative : each strand acts as a template for the synthesis of its partner, when replication is complete, there are two double helices each with one strand derived from parental dna molecule base paired with newly synthesized one -produce DNA DOUBLE HELICES THAT ARE HALF OLD AND HALF NEW semi-discontinuous: one strand continuous, other one is not -continuous strand reads dna in only one direction, from three prime to five prime leading strand -template strand of the DNA double helix -replication fork moves along it in the 3 prime to 5 prime direction -newly synthesized strand complementary to the original strand to be synthesized in the 5 prime to 3 prime direction (same direction as replication fork) lagging strand -strand of template DNA double helix -replication fork moves along it in 5 prime to 3 prime direction -orientation opposite to working direction of dna polymerase 3 (3 prime to 5 prime manner) 5. -proteins co
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