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Lecture 2

Lecture 2 Light outcomes.pdf

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Biology 1001A
Tom Haffie

To be used for energy or information light must be absorbed Lecture 2 Light Lecture Outcomes 1. Relationship between excited states of a pigment and its absorption, fluorescence emission spectra. 2. Region of the electromagnetic spectrum known as “visible light”.  400-700 nm 3. Relationship between wavelength and energy content of a photon.  Shorter the wavelength, stronger energy the wave has (inversely related) The amount of energy in the blue photon is higher than the amount of energy in the red photon. 4. Molecular characteristic of visible pigments that make them able to absorb light.  This conjugated (ring) system (the alternation of double bonds and single bonds) represents or indicates the specific kind of electron configuration; these are non-bonding electrons (Pi orbital electrons). Those electrons will interact with the photons of light. They are not required for bonding. 5. Relationship between pigments and associated protein.  Pigments are bound non-covalently to the proteins. When you isolated protein carefully enough, you can keep the pigments attached: pigment-protein complex. 6. Four “fates” of the excited state of chlorophyll resulting from absorption of photons.  Lose energy as heat Lose a little energy as heat, and then lose the energy as fluorescence Do work (photochemistry) Energy transfer 7. Reason(s) why relative fluorescence is different in isolated chlorophyll vs. intact cells when exposed to light.  Intact cell: the energy that is absorbed from exciting the electrons is used to power photosynthesis (and other essential processes in the cellDue to presence of protein complex and reaction centre in the intact cells, the energy re
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