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Lecture

Zab Methods Paper.pdf

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Department
Biology
Course
Biology 1001A
Professor
Tom Haffie
Semester
Fall

Description
Methods Three experiments were conducted to determine the effects of different ion 222sizesCaCl Ca BaClBa and MgClMgon the transformation of 22 2 Escherichia coli K12 by p600 12gL One molar of BaCl was diluted using 100 L of 1 M BaCl and 900 L of water 22to make 1000 L of 100 mM BaCl Using a P1000 micropipette 500 L of 100 mM 2CaCl BaCl and MgCl were added into 3 different 15 mL eppendorf tubes A line of 222E coli K12 from a plate was scraped using a sterile toothpick into each of the 15 mL eppendorf tubes All tubes were placed in ice for 2 minutes then each tube was split into two tubes with 250 L in each tube Ten microlitres of TE TrisEDTA was added to 3 tubes of each ionic solution Ten microlitres of p600 12 gL was added to the other three tubes The six tubes were placed in ice for 5 minutes heat shocked at 45
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