Class Notes (835,241)
Canada (509,043)
Biology (6,794)
Biology 1001A (1,727)
Tom Haffie (1,170)

Zab Methods Paper.pdf

2 Pages
Unlock Document

Biology 1001A
Tom Haffie

Methods Three experiments were conducted to determine the effects of different ion 222sizesCaCl Ca BaClBa and MgClMgon the transformation of 22 2 Escherichia coli K12 by p600 12gL One molar of BaCl was diluted using 100 L of 1 M BaCl and 900 L of water 22to make 1000 L of 100 mM BaCl Using a P1000 micropipette 500 L of 100 mM 2CaCl BaCl and MgCl were added into 3 different 15 mL eppendorf tubes A line of 222E coli K12 from a plate was scraped using a sterile toothpick into each of the 15 mL eppendorf tubes All tubes were placed in ice for 2 minutes then each tube was split into two tubes with 250 L in each tube Ten microlitres of TE TrisEDTA was added to 3 tubes of each ionic solution Ten microlitres of p600 12 gL was added to the other three tubes The six tubes were placed in ice for 5 minutes heat shocked at 45
More Less

Related notes for Biology 1001A

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.