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Lecture 11

Biology 1002B Lecture 11: Lecture 11 Integrated Metabolism II

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Biology 1002B
Denis Maxwell

Lecture 11: Integrated Metabolism II Thursday, February 16, 2017 11:00 AM • Measure photosynthesis as either the disappearance of CO2 or the appearance of oxygen (O2), they should be 1:1 • In Chalmydomonas, photosynthesis and cellular respiration are happening • In cellular respiration you have the reverse process occurring, as you are consuming oxygen • CO2 is consumed through photosynthesis and produced with cellular respiration Measuring Carbon Fixation • Fixing carbon into sugar in chamlydomonas (measuring photosynthesis/calvin cycle/carbon fixation/primary productivity) • Measuring CO2 in the gaseous phase • If there are lots of cells then the rate will obviously be higher, which is why we base the rate on a per cell basis Light Respone Curve • In the dark only cellular respiration is happening which means that nothing is taking the carbon dioxide that is being produced and the carbon dioxide rate is negative • Between 0 to 400 light intensity goes up linearly because the amount of ATP and NADPH goes up linearly with light intensity which means the carbon dioxide rate is limited to the ATP and NADPH rate • We can assume that regardless of light intensity, regardless of how much photosynthesis is going on, the rate of respiration is constant at whatever the dark rate is • For the plant to grow, it needs CO2 in which means you need to be at a light intensity that will bring it above zero, ie you want to be above the light compensation point in order for the plant to grow • If you're at the light compensation point, the rate at which photosynthesis is bringing in CO2 is perfectly offset at the rate the mitochondria is releasing CO2, which is why you want to be above this point Enzyme Kinetics • We want to measure the amount of product (P), the rate at which we are making product • You can link the production of product in the change in colour • The different purples have constant enzyme conc
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