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Lecture 8

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Biology 1002B
Tom Haffie

Lecture 8: Integrated Metabolism January, 30, 2013  If you add mitochondria to an oxygen electrode chamber what happens to the oxygen concentration in the chamber? o Measuring the rate of cellular respiration  From 0-2 minutes, nothing happens, no change  Time 2-4 add mitochondria it’s a linear line with a negative slope, time is independent, oxygen is dependent  Time 4-6 adding NADH, the rate of electron transport will go up, the beginning is substrate limited. The rate of respiration will increase. A linear line with a slope more negative than time 2-4  Time 6-8 adding ADP, the rate of respiration is going to increase, higher rate than time 4-6 when we added NADH. Therefore, the slope is even more negative slope  Time 8-10 adding a uncoupler, the oxygen consumption rate will increase even more, the highest consumption rate will be when we add a uncoupler, because there is no respiratory control, there is no electrochemical gradient, very easy to pump, so the rate of oxygen will be very high.  Respiratory control o The first 2-4 there is not a lot of NADH, so the rate is not as high, but after adding more NADH the cell will transport out more protons, so that the oxygen consumption rate will increase. When we don’t have ADP to consume the protons, electrochemical gradient will be greater and greater, and it will be harder and harder to pump electrons out. So
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