Biology 1002B Lecture Notes - Lecture 8: Light-Independent Reactions, Chlamydomonas, Electrochemical Gradient

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If you add mitochondria to an oxygen electrode chamber what happens to the oxygen concentration in the chamber: measuring the rate of cellular respiration. From 0-2 minutes, nothing happens, no change. Time 2-4 add mitochondria it"s a linear line with a negative slope, time is independent, oxygen is dependent. Time 4-6 adding nadh, the rate of electron transport will go up, the beginning is substrate limited. A linear line with a slope more negative than time 2-4. Time 6-8 adding adp, the rate of respiration is going to increase, higher rate than time 4-6 when we added nadh. Therefore, the slope is even more negative slope. Respiratory control: the first 2-4 there is not a lot of nadh, so the rate is not as high, but after adding more. Nadh the cell will transport out more protons, so that the oxygen consumption rate will increase.

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