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Lecture 8

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Western University
Biology 1002B
Tom Haffie

If you add mitochondria to an oxygen electrode camber, what happens to the oxygen concentration in the chamber?  Oxygen concentration as a function of time  0-2 seconds: ---  2-4 seconds; ATP added: \ (gradual decline)  Measuring the rate of oxygen consumption is a way of measuring the rate of cellular respiration  In the system, there is a hole in the top where you can use syringe to feed in molecules  4-6 seconds; NADH is added): rate of decline increases  Isolated mitochondria; the mitochondria will very quickly run out of substrate; there’s nothing to fuel it, but if you give it substrate, the mitochondria can transport the molecules in -> rate of electron transport goes up; initial rate is substrate-limited  8-10 seconds:  Respiratory Control  The rate of electron transport: the concentration gradient keeps it from going any faster  Without ADP to consume the proton, the gradient becomes very low; harder to pump it into a space that already has a lot of protons  The rate of electron transport is controlled by the availability of ADP  Rate is high if lots of ADP around, rate is low if not much ADP available  10-12 seconds: 2,4-dinitrophenol  The rate won’t be as steep; it inhibits ATP synthesis  No respiratory control  No pressure on the proton gradient, you don’t establish one at all  Every proton that moves across diffuses right back  You don’t establish any chemiosmosis  It becomes very easy to oxidize NADH  The rate of o
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