Biology 1002B Lecture Notes - Ribosomal Rna, Cyanobacteria, Protein Targeting
24 Aug 2013
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Document Summary
In order to determine the unknown vaucheria sequence, multiple sequences of related algae were aligned. This yields identifiable regions of high sequence conservation; sequences that correlate perfectly with that of vaucheria. Based on two regions of high sequence similarity, degenerate primers can be designed that encompass all the possible combinations of the identified dna sequences. The observed region of dna is then amplified. Transcript abundance refers to the amount of a certain mrna observed; in this case the transcript abundance of psbo. As the polymerase chain reaction (pcr) amplifies double-stranded dna, a more specific process known as reverse transcription pcr is needed to amplify single-stranded rna. The first step is to isolate the total rna (found in either elysia or vaucheria), which is mostly rrna as opposed to mrna. Next it is necessary to reverse transcribe that rna in order to obtain complementary. Dna (cdna), a single-strand piece of dna out of an rna template.
