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Lecture 11

Biology Lecture 11 Outcomes

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Biology 1002B
Tom Haffie

Lecture 11 The Electron transport chain  Occurs on inner mitochondrial membrane  NADH dehydrogenase (40 products of genes make it up)  Cytochrome C sits on the interbmembrane side of the membrane  Goal: o NADH donated to complex I and split into NAD and H o E- shuffles to Q and Cyt C, then to complex II…and so on Main Components of ETC:  NADH dehydrogenase (40 proteins)  Cytochrome complex  Cytochrome oxidase  Supramolecular complexes: many proteins come together to produce them *Product of ETC is nothing you need, where as in ETC of phyotosynthesis makes a product you need later on Why do electrons move?  Spontaneous flow  Organized from low to high redox potential (high reox pot= higher attraction to e-)  NADH has negative R potential  O has a very high R potential  Proteins have no ability to pull e off of NADH, they just hold cofactors in a specific way  The co factor on complex I has a higher affinity for e- than NADH  Oxygen: terminal e- acceptor of the chain (driving force!!) o Because its very electronegative, so it pulls e- down the chain o Oxidizing molecule Chemiosmosis  Is the link between e= transport and synthesis of ATP  Establishing a proton gradient across the membrane o Gradient represent potential energy that can be harnessed to do work  H+ accumulate in the inter-membrane space  Transport harnesses this energy  Proton Motive Force: how much energy is available  2 components o Concentration is different o Charge is different o PMF (mV) =  - 59(pH) o Mitochondria PMF= 220mV The electron transport chain: B  Energy that’s lost when you oxidized NADH is conserved thru proton pumping  Q takes up a H+ goes thru Q oxidation reduction cycle and puts it into the intermembrane space  Also happens at cytochrome oxidase  H+ flow back thru ATP synthase and produce ATP in the matrix  Oxidative phosphorylation: oxidizing the NADH and phosphorylating the ADP o Linking oxidation of NADH to activity of phosphorylation of ATP synthase o They are coupled processes o The proton gradient is what’s coupling the two  Each NADH3ATP  Each FADH2 2ATP o Only goes thru 2 complexes, it doesn’t go thru as much proton pumping  Oligomycin: prevents ATP synthesis thru oxidative phosphorylation (shuts it down) o Find out howww: Proton circuits versus electrical circuits  Only one path for e-
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