Biology 1202B Lecture Notes - Lecture 16: Nucleoside Triphosphate, Dna Replication, Deoxyribonucleotide
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Dna technologies: pcr, sequencing (sanger & genome) (chapter 15) The polymerase chain reaction amplifies dna in vitro. For replication to begin, a primer must be available, base-paired to the template chain: process: Denaturation heat dna containing target sequences to 95*c to denature it to single strands. Annealing cool the mixture to 55-65*c (depending on primers) to allow the two primers to anneal their complimentary sequences at the two ends of the target sequence. Extension heat o 72*c, the optimal temperature for dna polymerase to extend the primers, using the four nucleoside triphosphate precursors to make complimentary copies of the two template strands. Repeat the same steps of denaturation, annealing of primers, and extension in cycle 2, producing a total of 4 molecules. Repeat the same steps, producing a total of 8 molecules. Two of the eight match the exact length of the target dna sequence: note: The primers are made up of dna rather than rna like natural dna replication.