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Biology 1202B
Richard Gardiner

DNA Replication • DNA is replicated semi-conservativley – Meselson and Stahl used two different isotopes for Nitrogen ( N15 & N14) and added E. Coli to the medium - the lighter band is higher up and the darker band lower down - isolates DNA  see how the DNA settles - exam question – what would the other two options (conservative and dispersive) results look like? • DNA polymerase adds bases to 3’ Hydroxyl group but synthesizes 5’-3’ • Nucleoside triphosphate pairs – carbon sugar, phospahate group and a nucleotide base - only complimentary base will be able to join in • Phosphodiester linkage produces energy to break the covalent bond • Hydrogen bongs need to be strong enough to keep the helixes together but weak enough to be broken apart for replication • Bacterial polymerase—a clamp holds the DNA in place to allow more efficient/faster replication - if the clamp was not there, polymerase would polymerize and then disassociate making the process very slow •
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