Biology 1202B Lecture Notes - Covalent Bond, Nucleoside Triphosphate, Hydroxy Group
25 views1 pages
12 Feb 2014
School
Department
Course
Professor
Document Summary
Dna replication: dna is replicated semi-conservativley meselson and stahl used two different isotopes for nitrogen ( n15 & n14) and added e. coli to the medium. The lighter band is higher up and the darker band lower down. Isolates dna see how the dna settles. If the clamp was not there, polymerase would polymerize and then disassociate making the process very slow. Starts off with an origin of replication various binding protiens (cascade of molecules will start to process) Helicase unbinds the helix of dna energy dependant (hydrolyzed atp) Single stranded binding proteins proteins that bind to the individual strand to prevent it from becoming double stranded again. Topoisomerase nixes the backbone allowing it unwind releasing/relieving tension on the helixes. Rna primase recognizes origin of replication and lays down rna primer (3") attaches to clamp and starts putting bases down. No telomerase = shorter life span but less cancer.
Get access
Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers
Related Documents
Related Questions
1. | In addition to identifying the genetic material, the experiments of Avery, MacLeod, and McCarty with different strains of Streptococcus pneumoniae demonstrated that | ||||||||||
|
2. | In order to show that DNA in cell extracts is responsible for genetic transformation in Streptococcus pneumoniae, important corroborating evidence should indicate that _______ also destroy transforming activity. | ||||||||||
|
3. | Based on what you have learned about the experiments conducted by Griffith and Avery and colleagues with bacteria, which of the following would result in transformation of living R cells? | ||||||||||
|
4. | A-T base pairs in a DNA double helix | ||||||||||
|
5. | If 23 percent of the bases in a sample of double-stranded DNA are adenine, what percentage of the bases are uracil? | ||||||||||
|
6. | The uniform diameter of the DNA structure provides evidence for | ||||||||||
|
7. | If a sequence of one strand of DNA is 5â²-TGACTATC-3â², what is the complementary strand? | ||||||||||
|
8. | What structural aspect of the DNA facilitates dissociation of the two DNA strands for replication? | ||||||||||
|
9. | If the MeselsonâStahl density gradient experiment had resulted in two bands of DNA molecules after only one round of replication, one containing only 15N and the second only 14N, this result would have indicated that replication was | ||||||||||
|
10. | The nucleoside analogue acyclovir, which is used to treat herpes simplex virus (HSV) infections, lacks a 3â² hydroxyl group (âOH). Predict what will happen if the host cell DNA polymerase incorporates a molecule of acyclovir into an elongating strand of HSV DNA. | ||||||||||
|
11. | Which of the following does not demonstrate the stability of the DNA double helix? | ||||||||||
|
12. | What effect would a primase inhibitor have on DNA replication? | ||||||||||
|
13. | To replicate their DNA in a timely manner, most eukaryotic chromosomes | ||||||||||
|
14. | Which statement about DNA replication is false? | ||||||||||
|
15. | In many eukaryotes, there are repetitive sequences called telomeres at the ends of chromosomes. After successive rounds of DNA replication, the _______ strand becomes shorter. In some cells, an enzyme called _______ repairs the shortened strand. | ||||||||||
|
16. | A researcher studies normal human fibroblast cells. They can be maintained in culture but die off after about 30 cell generations. Unexpectedly, a colony of cells continues to survive and divide past 30 generations. Which scenario is most likely true for these cells? | ||||||||||
|
17. | If DNA polymerase III introduces an incorrect nucleotide, what is the first corrective action made by the DNA repair system? | ||||||||||
|
18. | Choose the correct order of the following four events in the excision repair of DNA: (1) Base-paired DNA is made complementary to the template. (2) Damaged bases are recognized. (3) DNA ligase seals the new strand to existing DNA. (4) Part of a single strand is excised. | ||||||||||
|
19. | Six complete cycles of PCR should result in a _______-fold increase in the amount of DNA. | ||||||||||
|
20. | When double-stranded DNA is heated to temperatures above 90°C, it denatures. Denaturation is a process that | ||||||||||
|