Class Notes (838,033)
Canada (510,626)
Biology (6,824)
Lecture

2012.03.07 - Bio 1202 Lecture Review Notes.docx

2 Pages
136 Views
Unlock Document

Department
Biology
Course
Biology 1202B
Professor
Gardiner/ Murphy
Semester
Winter

Description
Biology Lecture Review Notes Lecture 6 DNA Technologies  Biotechnology - Technology applied to biological systems or living organisms to make or modify products or processes for a specific purpose o Genetic engineering – manipulation of genetic material  Bacterial Restriction Enzymes or Restriction Endonucleases (RE) o RE recognize short amount of DNA sequence (4-8nt long) called restriction sites o Restriction sites are often palindromes - same sequence 5’ to 3’ on both strands  Example: 5’GATC 3’ and 3’CTAG 5’ o RE cut DNA within these restriction sites o Complete digestion - conditions are such that if a restriction enzyme site is present in the dsDNA, the enzyme will cut at all the dsDNA sites o Incomplete digestion - conditions are such that if a restriction enzyme site is present in the dsDNA, the enzyme may cut at all the dsDNA sites  Conditions include things like temperature, amount of time, etc. o Sticky end – can bind back together after being cut by a RE o Another DNA fragment (complementary to the strands) from EcoRI digestion can bind to these sticky ends, but need DNA ligase to bind it all together (seal the nicks) o Blunt cutter – cuts straight through DNA and does not create a sticky end  Clones – a line of genetically identical cells or individuals derived from a single ancestor o Cloning – older method of amplifying cells and gaining identical daughter cells for further studying o Cloning a DNA fragment into a bacterial plasmid  Isolate gDNA and RE cuts it into fragments  The same RE cuts a bacterial plasmid  Mix the cut gDNA with the cut plasmid (they have the same sticky ends)  This creates recombinant DNA, which is inserted into a bacterial cell  The bacteria grow and divide (replicates and amplifies the gDNA)  Identify the bacteria with the gDNA of interest and grow it to produce more o Low transformation efficiency – the majority of bacterial cells do not take up a plasmid o Plasmids are modified into a cloning vector (something that can accept foreign DNA) o How to determine which bacterial cells have modified plasmids  Selection - insertion of genes to identify which bacteria have a modified plasmid  Give plasmids ampicillin resistance gene, so it can live in ampicillin  Bacterial cells with a modified plasmid will grow in ampicillin  Bacterial cells without a modified plasmid will not grow in ampicillin  This will eliminate all the plasmids without fragments  Screening - genes to identify whi
More Less

Related notes for Biology 1202B

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit