Biology 2382B Lecture Notes - Lecture 5: Scanning Electron Microscope, Förster Resonance Energy Transfer, Confocal Microscopy
18 Oct 2016
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Intro: mechanical fragmentation: grind cells or blend them; there will be some damage, trypsin: enzyme (protease) that breaks down the cell-cell matrix to release individual cells, edta: calcium chelator breaks the cell-cell matrix by degrading calcium. Growing culture needs: nutrients (amino acids, vitamins, minerals, glucose and salts) serum for cell division (found in blood insulin, growth factors), 37oc co2 incubator with 5% co2 (present in tissues). *serum can be derived from other animals as well; typically bovine (cow) serum is used in labs. Primary cell culture: cell taken directly from an organism (donated, post-mortem, etc. ) Cell line: cells that are transformed and grow indefinitely less likely to express contact inhibition. Cultures grown in adherent cell cultures or suspension cell cultures. Transformed cells: immortal and less likely to express contact inhibition; loss of hayflick limit (normal cells are capable of about ~50 divisions) cancer cells.
