Class Notes (837,548)
Canada (510,312)
Biology (6,824)
Lecture

Lecture 1.docx

3 Pages
60 Views
Unlock Document

Department
Biology
Course
Biology 2382B
Professor
Robert Cumming
Semester
Winter

Description
Lecture 1: Introduction to Cell Bio What is Cell Biology? - Cell biology is an academic discipline that studies, the basic structural, functional and biological unit of all known organisms - Anything that tis alive is composed of cells - Cell biologists looks at all aspects of the cell  Cell structure  Cell organelles and membrane trafficking  Cell cycle, division and death  Cell movement  Cell signaling  Cell communication Why study cells? - Part of the core in biology – we only know a small fraction about cell - Fundamental unit of life - Many unanswered questions - Storehouse for thousands of genes – there is symbiotic relation between genes and cell - Determine what is “normal” so we can fix the “abnormal” – MEDICLA IMPLICATION - Lead to Nobel Prize What is a Cell? (Fig 1.12) - Electron Micrograph – PRIMARY FOCUS ON EUKARYOTIC CELLS – i.e. once w/ nucleus - Ultimate Goal: to understand how macromolecular systems and organelles work and cooperate to enable cells to function autonomously (alone) and in tissue (w/ other cells) How do we study cells? Hypothesis Drive Experiments - First, you’ll need the tools and methods to isolate and maintain cell in vitro, to know how to view cells and what to look for, how to separate cell organelles and finally how to identify and study how proteins drive a cell biological process - Once you have the tools, we can start to ask, and more importantly begin to answer, biological questions pertaining to our ~ 75 trillion cells - In vitro: outside the body in an artificial state Cell Culture - Technique used to grow cells or tissues outside the organism under strictly controlled conditions.  To understand cell- we need to isolate the cells first before they are grown and analysed (in a realistic manner) - 1 we need to breakdown the cell-cell and cell-matrix interactions  Cells stick together – need to separate them  Mechanical fragmentation - cut w/ sissors into smaller chunks  Pushed through a mesh – makes clumps fo cells  TRIPSIN – enzymic breakdown (protease) which chews up the extracellular protein from stickinf to each other  EDTA – suppresses calcium which is required for extracellular proteins to function – so by preventing their function prevents cells from sticking - 2 we need the right conditions for the plated cells to grow  We need to regulate  temp (37)  O2 levels  Flow of nutrients  We need to mimc conditions inside the body in vitro  Cells are supplied w/ proper nutrients (amino acids, minerals vitamins, salts, glucose, etc.  Also need to introduce Co2 to the culture  Serum is added –blood prodict from cows – devoid o
More Less

Related notes for Biology 2382B

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit