Biology 2382B Lecture Notes - Lecture 14: Geometer Moth, Elastic Energy, Spindle Apparatus

• School: Western University
• Department: Biology
• Course: Biology 2382B
• Professor: Robert Cumming

Document Summary

There are three common classes of myosin, which all move toward the plus-end of actin filaments. Myosin i consists of a head domain with a variable number of light chains associated with the neck domain. Members of the myosin i class are the only myosins to have a single head domain and associate directly with membranes through lipid interactions (like in endocytosis). Myosin ii proteins have two head domains and two light chains per neck and are the only class that can assemble into bipolar filaments. Myosin v proteins have two head domains and six light chains per neck. They bind to specific receptors on organelles, which they transport (also involved in transport of endocytic vesicles). A sliding filament assay can be used to detect myosin-powered movement after myosin molecules are absorbed onto the surface of a glass coverslip and the excess unbound myosin is removed.