Biology 2382B Lecture Notes - Lecture 2: Vacuole, Hat Medium, Plasmid
22 Mar 2018
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Learning objectives: bright-field microscopy and resolution, phase-contrast microscopy, differential interference contrast microscopy, fluorescence microscopy, gfp and fluorescent fusion proteins, confocal microscopy, deconvolution, fret, electron microscopy. In order to see detail, you need contrast: refractive ability of specimen is important for seeing that detail, magnification = product of objective and ocular lenses together e. g. objective is 100x, ocular is. 10x magnification is 1000x: maximum magnification with conventional light microscope is 1000x. Resolution of microscopes: resolution: ability to distinguish between two very closely positioned objects as separate entities, conventional light microscope usually cannot resolve objects/cellular features that are less than. Lens with high numeric aperture (can cost 5 to 10 thousand dollars to engineer: the best resolution for a light microscope is 200nm, do not (cid:272)all it (cid:862)high resolutio(cid:374)(cid:863) (cid:272)all it (cid:862)opti(cid:373)al resolutio(cid:374)(cid:863) If you artificially slow down wave by a quarter wavelength, you can align it to create dimmer cells within cells.
