Biology 2382B Lecture Notes - Lecture 15: Dichroic Filter, Fluorescent Lamp, Hat Medium

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How are Fluorescence Images Obtained?
- Light from light source must be focused and filtered by the excitation filter in order to have a beam of
light that is at optimal excitation wavelength (ex. green light).
- Light then hits the dichroic mirror which reflects the excitation light causing it to go down through the
objective lens and strike the specimen on the microscope stage.
- It excites the fluorophores within a specimen and the emitted light (ex. red light) will pass through the
objective lens and b/c the emitted light is of a larger wavelength, it will pass through the dichroic mirror!
- The red light will then pass through a projection lens and then to an emission filter to be processed
(emitted light is a range, so emission filter filters out the light to just the optimum emission wavelength).
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Document Summary

Light from light source must be focused and filtered by the excitation filter in order to have a beam of light that is at optimal excitation wavelength (ex. green light). Light then hits the dichroic mirror which reflects the excitation light causing it to go down through the objective lens and strike the specimen on the microscope stage. It excites the fluorophores within a specimen and the emitted light (ex. red light) will pass through the objective lens and b/c the emitted light is of a larger wavelength, it will pass through the dichroic mirror! The red light will then pass through a projection lens and then to an emission filter to be processed (emitted light is a range, so emission filter filters out the light to just the optimum emission wavelength). B-lymphocytes generate antibodies as a response: harvest specific spleen cells.

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