Biology 2382B Lecture Notes - Lecture 3: Electric Field, Amphiphile, Phospholipid
10 Feb 2014
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Lecture 3: isolation and analysis of cell organelles and molecules. Labeling live cells with fluorescent antibodies or stains. Antibodies are used to isolate pure fractions of cell: note: fluorophore-linked secondary antibodies could also be used in combination with unlabeled primary antibodies. Fluorescent activated cell sorting (facs: cells pass single file through a laser light beam, both fluorescent light emitted and scattered are measured by detectors. Individual cells are forced through a nozzle and given a charge proportional to the fluorescence detected degree of: cells with different electric charges are separated by an electric field and collected. Facs can both analyze the cells and select one or few cells from thousands of them into a separate culture dish. To sort the cells, their concentration has to be adjusted so that the tiny droplets that the. Facs machine makes and analyzes contain only one cell each.
