Biology 2382B Lecture Notes - Chloroplast, Scanning Electron Microscope, Electric Field
12 Feb 2014
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3: isolation & analysis of organelles & molecules. Image acquisition: confocal fluorescence microscopy illuminates only a focal plane of the specimen and achieves greater clarity than conventional fluorescence microscopy, which produces a blurry image due to fluorescence detection above and below the focal plane. Deconvolution microscopy: deconvolution algorithm) that provides an alternative means of producing high-resolution images. is a computational restoration method (using the point spread function. In deconvolution focal (merkez) planes called a z-stack confocal microscope. microscopy. Fluorescence resonance energy transfer (fret) is used as method to measure protein interactions in live cells. If there is an interaction, the use of two filter sets will detect the two possible wavelengths of the fluorescent proteins. Though fluorescence microscopy has many advantages (uses live cells, sees cellular structures), it is somewhat limited by its resolution, partly because of the limited properties of light microscopy (between 300 and 700nm).
