Biology 2382B Lecture Notes - Dynactin, Axoneme, Basal Body

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Vesicle transport (bi-directional: motor proteins require energy (atp, using squid axons is one of the best models for tracking movement. Section 2: squids have large nerves (up to 1 mm in diameter, squid axons are easy to remove, easy to extrude can get the cytoplasm out of this squid nerve and observe things moving around. Things of different sizes can move in different directions, in different speeds: not random things move on specific railways at specific rates. Labeled proteins travel at different speeds in cells (not diffusion: radioactive amino acids are injected into a nerve body such that the proteins that the nerve body makes will be radioactive. After about an hour, the cell has made proteins; because it is an axon, some of the proteins get transported along that axon. Axon is divided into four regions representing different distances from where the amino acids were injected.

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