Biology 2382B Lecture Notes - Lecture 3: Polyacrylamide, Sonication, Electrophoresis
Document Summary
Sorts cells into separate culture dishes via an electromagnetic field and labeling cells with electric charges based on fluorescence detected. Hypotonic solution- lower ion concentration draws water into the cell. Produces mix of suspended cellular components, the homogenate. Differential centrifugations means centrifuging a homogenate at different speeds and times. Usually carried out in presence of negatively charged detergent, sds. Detergent dissociates multimeric proteins and denatures all polypeptide chains. Mobility of sds-protein complexes are influenced by molecular size; proteins separate into bands. Bands from sds-page are transferred/blotted from gel onto porous membrane.