Biology 2382B Lecture Notes - Lecture 23: Protein Kinase C, Insulin Receptor Substrate, Cgmp-Dependent Protein Kinase
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This pathway can be triggered by a ligand binding to a gpcr that acivates either the g o or g q subunit leading to the acivaion of phospholipase c. cleavage of plp2 by phospholipase c yields ip 3 and dag. Ater difusing through the cytosol, ip3 interacts with and opens ca2+ channels in the membrane of the endoplasmic reiculum, causing the release of stored ca2+ ions into the cytosol. One of several cellular responses induced by a rise in cytosolic ca2+ is the recruitment of protein kinase c (pkc) to the plasma membrane, where it is acivated by dag. The acivated membrane-associated kinase can then phosphorylate various cellular enzymes and receptors, thereby altering their acivity (step 6). The ca2+ nitric oxide (no)-cgmp pathway is important for the relaxaion of arterial smooth muscle. Nitric oxide is synthesized in endothelial cells in response to the acivaion of acetylcholine gpcr, phospholipase c, and the subsequent elevaion in cytosolic ca2+.