Biology 2382B Lecture Notes - Lecture 2: Organelle, Fluorophore, Bioluminescence

• School: Western University
• Department: Biology
• Course: Biology 2382B
• Professor: Sashko Damjanovski

Document Summary

Microscopy is based off the concept that samples bend or refract certain wavelengths of light. = uses a microscope with more than one lens. = uses a microscope that projects light onto the sample from below using just a typical light bulb. Ocular lens = the one you look through. Objective lens = the one closer to specimen. Only structures with a high refractive index (high ability to bend light)are observable. = the ability to distinguish between two very closely positioned objects as separate entities. Larger resolution = smaller d is better. Traditional resolution loss for a microscope is at 0. 2um - this means that features of a sample need to be >~0. 2um apart in order to be distinguished on a conventional microscope. Typically, this means traditional microscopes can get down to the cellular level, but we need electron microscopy to get down to the organelle level. D = distance resolved between 2 points.