Biology 2382B Lecture Notes - Lecture 12: Dynactin, Axoneme, Kinesin
28 Apr 2016
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Document Summary
Microtubules are tracks for transport, and allow bi-directional vesicle transport this can be endocytosis or exocytosis. This uses motor proteins which require atp: this means that with no atp, there will be no movement. Using a squid axon as a model system, they discovered axonal transport: they did so by first injecting radioactive amino acids such that the nerve ganglion could create labelled proteins. They found that the labelled proteins travel at different speeds in the cells: they injected these proteins into the beginning of many squid axons. By exposing the gel to x-rays, they could visualize each group of protein that ran together (i. e. transported together along the axon) was by the banding pattern. Kinesins mostly move to towards the + end (e. g. fungi have microtubules that move towards the end), such that they are microtubule + end directed motor proteins. There are many different types 14 known classes with 45 genes in humans.
