Biology 2581B Lecture Notes - Lecture 11: Transcription Activator-Like Effector Nuclease, Thymus, Zygosity
14 Mar 2017
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Previously needed to rely on spontaneous mutations to occur, or randomly induced. Today can generate mutants with a specific mutation in a specific place. Transcription factor mutation in the arabdopsis plant: ex. Drosophila white eye mutation (first sex-linked mutation discovered in fruit fly: ex. Mice short tail mutation (heterozygous will be mutant and homozygous will be lethal due to: ex. Mutated (cid:498)nude(cid:499) mouse lack a thymus and cannot generate immune response: disruption of: ex. C elegans worm mutation shorter, longer, thicker defect in mesoderm formation) Problem: there are too few spontaneous mutations, and too slow: need mutants with not just phenotypic changes. Can do assays: take part of each individual plant leaf and look at proteins, developmental mutants, mutants with different behaviour patterns, mutants which react different to environment: light, salt, toxins, wind, touch . Build a collection of mutants and identify one with same phenotype.
