Biology 2581B Lecture Notes - Lecture 23: Map2K2, Syncytium, Morphogen
22 Apr 2017
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Document Summary
How does one gene affect the expression of another: you can follow expression patterns. Example: in drosophila, imaginal discs are an area which develops into wings. Wingless is required for proper vestigial positioning within the development of imaginal discs. Expression of vestigial depends on the presence of wingless, and it has to be in neighbouring cells. By looking at expression patterns we can find out a lot about these two proteins, and what impact they have on each other: one is required for the other"s positioning. Imagine we have mutant that has a knockout, with its associated phenotype. Compare double mutant phenotype to single mutant phenotype. We can also analyze a bunch of double mutants. We are most interested in number 4. If you do a double mutant, they could possibly be acting in two different pathways. If double mutant only shows phenotype of a or b, then we know that b is potentially epistatic to gene a.
