Biology 2581B Lecture Notes - Lecture 9: Chain Reaction, Chromatography, Northern Blot
25 Feb 2014
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Lecture 8: select genetic technologies: basic molecular technologies. 1953 discovery of dna structure: james watson and francis crick, maurice hugh frederick wilkins, rosalind franklin (her data was used by. Early 1970"s restriction enzymes, arber, nathans, smith. 1983 pcr, kary mullis: mid 1980"s rna editing, simultaneously by several groups. 1976 viral, 1995 bacterial & eukaryotic, 2003 human genome. 2000 and up new sequencing technologies, digital pcr large scale screening methodologies. Restriction enzymes: restriction enzymes cut dna molecules at specific locations to produce restriction fragments with either blunt or sticky ends, restriction enzymes have, specific recognition sequences, can differ in length, result in specific cutting patterns. Fragments fit only back together if they have: same type of ends, matching sequences. Enzymes have different recognition sites but they generate the same overhangs. The number of base pairs in a recognition site determines the average distance between sites in a genome and thus, the size of fragments produced.
