Biology 3597A/B Lecture Notes - Lecture 15: Heteroduplex, Drosha, Exon
16 Mar 2017
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This is not alternative splicing, this is post transcriptional modification of bases by different enzymes and proteins. Looked at trypanosome mitochondrial genome (relatively simple for mitochondrial genome cause those genes also move to nucleus) Found mrna was different, added and deleted uracils primarily addition. This is an important gene part of electron transport chain critical to survival. Not random insertions and deletions, but also these alterations were functional correct internal frameshifts, some create initiation codons. Tra(cid:374)s(cid:272)ri(cid:271)ed (cid:271)ut do(cid:374)"t e(cid:448)e(cid:374) ha(cid:448)e i(cid:374)itiatio(cid:374) (cid:272)odo(cid:374) till edited. Other genes in genome which serve as guides for edited genes. Edited genes will have separate gene coding for guide rna which will play a role in adding or removing nts specificity. Template for edited version which binds to non edited version (guides) In order for edited deletion, you need guide rna. If you get insertion, you need 2 things, 1-guide rna 2-utp u tri phosphate you need.
