Biology 4218A Lecture Notes - Centromere, Mitosis, Alpha Cell

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Haploid easy to get mutants and mutate (oomycetes diploid) Convenience factors (size, cost, rapid life cycles) Analysis of individual meiosis possible, easy to analyze mitotic recombination. Wide range of gamete types (isogamy to anisogamy) Homothallism: one strain sufficient for sexual development. Heterothallism: two strains needed for sexual development (opposite mates) Secondary homothallism: separate mating types (sexual spores formed with both present) Vegetative incompatibility groups (vcg) can prevent fusion of vegetative hyphae block sexual reproduction. Mating type systems: bipolar: involves 1 locus with 2 to many alleles, tetrapolar: involve 2 loci each with 2 to many alleles (distinct functions) Example: a2b3 can mate with a1b5 (must be different at both a and b for full mating) Analysis of a new fungus: isolate mutants, mutagenize, select mutants (devise screen i. e. plate on drug containing medium for drug resistance, identify mutant if necessary (i. e. what growth factor does nutritional mutant need?)

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