Biology 1001A Lecture Notes - Lecture 6: Thymidine Triphosphate, Dna Ligase, Primase

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Watson and crick brought together information from several sources to work out. Each dna is made of four nucleotides. Each nucleotide is a five carbon sugar (deoxyribose), a phosphate group and one of the four nitrogenous bases (a,c,g,t) Adenine and guanine are purines, cytosine and thymine are pyrimidines. Sugar phosphate backbone is formed for dna, through phosphodiester: purine= fused carbon rings, pyrimidine= single carbon ring. Chargaff"s rule: a=t and c=g (amount) bonding (cid:523)phosphates connecting 3" and 5" ends of carbon rings(cid:524) (cid:498)complementary base pairing(cid:499) Bases extend to fill the empty space between the two backbones. A purine bonded with a pyrimidine are wide enough to fill that space. Base pairs are connected by hydrogen bonds. A full turn contains 10 base pairs. The new model proposed that two polynucleotide chains wind into a dna double helix. Semiconservative replication (watson and crick) the parent dna. Hydrogen bonds break allowing the strands to unwind.

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