Bio lectures 6-10.doc

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14 Nov 2012
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BIO LECTURE 6- WED SEPTEMBER 29TH
EUKARYOTIC RECOMBINATION- SEE FIGURE 12.12 P 261
Clicker Questions: My son jay as a unique combination of alleles as a result of “cutting
and pasting” DNA backbones. When did this cutting and pasting occur?
a) when he was a newly fertilized zygote
b) when he was a developing fetus
c) When he reached sexual maturity
d) none of the above
ANSWER: D
Which type of bacterial genetic exchange involves donor cells that are dead?
a) conjugation
b) transduction
c) transformation
ANSWER: B AND C
RECAP: Inheritance of Difference in Prok
-mutations arise
-bacterial DNA can be moved from Donors to reciepients
-DNA can be incorporated into a recipient through recombination
-some genes are mobile so can be transferred to another site then to another host
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-transposon aka jumping genes or mobile elements
-F Factor is a plasmid that codes only for its own replication
-this is even smaller, just the red part, a little bit of DNA all it does it code for its own
replication and transfer
-codes for enzyme called transposee
-it sends a copy of itself from the bacterial chromosome to the other red part on the
plasmid
-ex of a replicative movement (sends a copy of itself to another site)
-the red part is the transposon, the yellow part is an antibiodic resistance gene
-so now this plasmid can transfer this gene to a recipient cell
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-plasmid is transferred through conjugation to a recipient cell
-during fertilization, genes from two different organisms are brought into close proximity
and can recombine
-a zygote has DNA from two different parents and can therefore cross over and
combine (to make gametes, not to make the rest of the body cells)
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