Biology 1002B Lecture Notes - Lecture 1: Insertional Mutagenesis, Chlamydomonas, Bleomycin
15 Jan 2017
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Diverged early from plants share characteristics with them (ie. photosynthesis) Close to the base of the evolutionary tree (diverged over 1 bya) Shares common traits with humans and plants. Flagella shares homology with cilia in lungs. Relationship between genome size and protein coding genes. Human genome is significantly bigger than the chlamy genome, but only have 5,000 more coding genes than them. Zygotes in animals go through mitosis to give a human body. In algae and fungi, zygotes go through meiosis to make spores. Zoospores reproduce asexually by mitosis and give rise to + and (2 different mating types) Chlammy cells divide about once every 8 hours. Under stress, zoospores will behave like gametes again to create a zygote and reproduce sexually. Basics of insertional mutagenesisforeign dna, electroporation, plating on agar plates What is bleomycin, how is it used in the isolation of mutants. Characteristics of chlamydomonas that make it a useful model system.
