Biology 1002B Lecture Notes - Lecture 10: Pyruvic Acid, Reduction Potential, Acetyl-Coa
27 Feb 2017
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Change in respiration rate (oxygen consumption) in isolated mitochondria by addition of nadh, If you add mitochondria to an oxygen electrode chamber, what happens to the oxygen concentration in the chamber: it will decrease in concentration as the mitochondria is using up the oxygen. 2 minutes- add mitochondria: add mitochondria, oxygen consumed, rate will go down. 4 minutes- add nadh: rate of electron transport goes up, add nadh which feeds mitochondria substrate; adding substrate, oxygen consumption goes up (slope is. Steeper, increases: more substrate to fuel electron transport. 8 minutes- add uncoupler (2,4-dinitrophenol): rate goes up even more (highest: protons diffuse across membrane, puts holes in the membrane; no proton gradient established over time, membrane is incredibly leaky to protons due to holes. Don"t care about where on the y axis the line is, only care about the slope of the line. This is where most of the atp is made.
