Biology 1002B Lecture Notes - Lecture 8: Light-Independent Reactions, Chlamydomonas, Electrochemical Gradient

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Published on 15 Apr 2013
School
Western University
Department
Biology
Course
Biology 1002B
Professor
Lecture 8: Integrated Metabolism
January, 30, 2013
If you add mitochondria to an oxygen electrode chamber what happens to the oxygen
concentration in the chamber?
o Measuring the rate of cellular respiration
From 0-2 minutes, nothing happens, no change
Time 2-4 add mitochondria it’s a linear line with a negative slope, time is
independent, oxygen is dependent
Time 4-6 adding NADH, the rate of electron transport will go up, the beginning is
substrate limited. The rate of respiration will increase. A linear line with a slope
more negative than time 2-4
Time 6-8 adding ADP, the rate of respiration is going to increase, higher rate
than time 4-6 when we added NADH. Therefore, the slope is even more
negative slope
Time 8-10 adding a uncoupler, the oxygen consumption rate will increase even
more, the highest consumption rate will be when we add a uncoupler, because
there is no respiratory control, there is no electrochemical gradient, very easy to
pump, so the rate of oxygen will be very high.
Respiratory control
o The first 2-4 there is not a lot of NADH, so the rate is not as high, but after adding more
NADH the cell will transport out more protons, so that the oxygen consumption rate will
increase. When we don’t have ADP to consume the protons, electrochemical gradient
will be greater and greater, and it will be harder and harder to pump electrons out. So if
there is a lot of ADP, the rate will be higher, there will be less gradient, and thus more
electrons will be readily pumped out, so more oxygen will be consumed. Therefore
In Chlamydomonas there are two ATP generating organelles mitochondria and chloroplasts in
one cell. Compared to animal cells how is mitochondrial ATP production in Chlamydomonas
different
o A. it is not different
o B. ATP is generated only when chloroplast ATP is low
o C. ATP is not made, just NADH
o D. ATP is not made instead the mitochondrion is the site of critical biosynthetic
reactions
ATP synthesized in chloroplast does not leave the chloroplast, no left overs to
be exported, all goes to Calvin cycle, making macromolecules. The mitochondria
behave the same as animal mitochondria.
The product of Calvin cycle (G3P) can be exported into the cytosol, can be made
into glucose, it is also an intermediate of glycolysis (pyruvate), so it can be
transferred into mitochondria and move into the CAC
The carbon can be used to make: amino acid, fats, molecules, etc.
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Document Summary

If you add mitochondria to an oxygen electrode chamber what happens to the oxygen concentration in the chamber: measuring the rate of cellular respiration. From 0-2 minutes, nothing happens, no change. Time 2-4 add mitochondria it"s a linear line with a negative slope, time is independent, oxygen is dependent. Time 4-6 adding nadh, the rate of electron transport will go up, the beginning is substrate limited. A linear line with a slope more negative than time 2-4. Time 6-8 adding adp, the rate of respiration is going to increase, higher rate than time 4-6 when we added nadh. Therefore, the slope is even more negative slope. Respiratory control: the first 2-4 there is not a lot of nadh, so the rate is not as high, but after adding more. Nadh the cell will transport out more protons, so that the oxygen consumption rate will increase.

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