• Chlamydomonas is a haploid single cell eukaryote, it can move around
with its flagella
- Has mitochondria, chloroplast, nucleus
- Way bigger than a bacteria
• Eukaryotic cell is much bigger than prokaryotic cell
• What makes chlamydomonas a “model system”?
• It uses light in two different ways
- Use light as a source of energy, to drive metabolic reaction
- Use light to sense environment like eye
- It is easy to grow
- Chlamy is a model system because it can do Mixotrophic growth
- Chlamy as a model system because it has chloroplast and
mitochondria, and Chlamy’s mitochondria functions the same as the
one in animal cell
• Eyespot is in the chloroplast but has nothing to do with photosynthesis
Lecture 1: chlamydomonas is a haploid single cell eukaryote, it can move around with its flagella. Use light as a source of energy, to drive metabolic reaction. Use light to sense environment like eye. Chlamy is a model system because it can do mixotrophic growth. In the nucleus, nuclear genome (what ppl take about most of the time) Mitochondrial genome: photoautotrophs, means photosynthetic organisms, arabidopsis is the model plant, synechsystis is a prokaryotic cell, it is a bacteria with no nucleus, but it is photos(cid:455)(cid:374)theti(cid:272). The easiest approach is to find an organism in which the trait is not (cid:449)orki(cid:374)g, a(cid:374)d look at the differe(cid:374)(cid:272)e (cid:271)et(cid:449)ee(cid:374) the (cid:862)(cid:373)uta(cid:374)t(cid:863) a(cid:374)d the (cid:862)(cid:374)or(cid:373)al(cid:863). 2: a mutated gene is a change in the double stranded sequence comparing to wild type. It would take forever for you to find a mutated cell in the wild: so its better just make our own mutants, make a population of mutants and look at the difference.