Biology 1002B Lecture Notes - Lecture 4: Confocal Microscopy, Superoxide, Mitochondrion
Document Summary
Indo, hiroko p. , et al. (2007) evidence of ros generation by mitochondria in cells with impaired electron transport chain and mitochondrial dna damage. Mtdna-lacking cells and mtdna deletion cells were studied with five different etc inhibitors using a fluorescent dye to detect hydroxyl radicals and a laser confocal microscope detection method. Overall it was found that the etc inhibitor treatment increased intracellular reactive oxygen species (ros). The majority of the fluorescent dye used was localized in the mitochondria, indicating that the main source of intracellular ros is in the mitochondria.