Biology 1002B Lecture Notes - Lecture 11: Sanger Sequencing, Massively Parallel (Computing), Crispr
3 Feb 2020
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Slide 1: crispr changes everything: clusters of regularly interspread short palindromic repeats. Find organism with smallest genome: what genes are in common in all life, an evolutionary scan to see what genes are common and start knocking them out. Start knocking them out, one by one, to see which ones are essential. Sequencing: annotation, cost of sequencing is dropping significantly. Slide 7: dna sequencing methods typically have: purification, assembly of fragment sequences. Slide 8: whole genomes can be sequenced by shotgun strategies: millions of copies broken into random pieces, this only works because we have millions of copies to start with. Sequence each fragment and reassemble into one continuous sequence. If it gets incorporated into a growing strand then synthesis stops: dna sequencing is actually dna synthesis, they block elongation of a growing strand. Slide 10/11: sanger sequencing uses four different reactions.
