Biology 1002B Lecture Notes - Lecture 8: Competitive Inhibition, C4 Carbon Fixation, Rubisco
9 Mar 2016
School
Department
Course
Professor
Document Summary
Grind up chlamy -> purify sample (only intact mitochondria in buffer) -> add to chamber. No more proton gradient (really, really easy to pump protons since there is no more pressure), electron can continuously flow down the chain. Proton gradient established (more nadh can be oxidized, easier to pump protons across the membrane) Makes its own food (light and co2) in the chloroplast. Atp also consumed by the calvin cycle. But we know that chloroplast exports energy to the rest of the cell. G-3p (every 3 turns of the calvin cycle makes 1) Bring in c-h bonds (food) from the external environment. Chlamy does have an acetate transporter though. Can completely grow in acetate (no light and co2) Also has one attach to the mitochondria that converts acetate into acetyl-coa. Grows best when there is light, co2, and acetate available. Umol of co2 consumed per minute per cell. Rate where co2 can be fixed into glucose.
