Biology 1201A Lecture Notes - Antonie Van Leeuwenhoek, Electron Microscope, Robert Hooke
1 Aug 2013
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Early microscope: anthony van leeuwenhoek single lens ca. The cell: first person to coin the word cell to describe the tiniest components of living systems plants (robert hooke) In modern telescopes, dyes are used to see ultraviolet rays. Different images include surface image and cross image. Resolution limit of light microscope is about 0,2 microns (size of a small bacterium, remember 1 micron is . 001 mm) In 1950"s, the introduction of the electron microscope enabled organelles, viruses, proteins, etc to be imaged. The minimum resolution of a light microscope is about 2 microns, the size of a small bacterium. Light microscopes can magnify effectively to about 1000 times the size of the actual specimen. Take cells apart to study their components. Centrifuge is used to fractionate cells and separate their major organelles. Ultracentrifuges are capable of speeds as fast as 150,000 rpm applying forces over 1 million times the force of gravity.
