Biology 1202B Lecture Notes - Lecture 16: Mycoplasma Genitalium, Plant Cell, Green Fluorescent Protein
15 Mar 2017
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If we see all red - we know that we have all of the cancer cells. If we see all green - we see normal dna. Labelled dna applied to array: all common snps in a genome can be assessed on a single chip, snps account for a large portion of the variation between individuals, serve as important genetic markers. > hybridization: you perform a microarray experiment with cdna from normal cells labelled with cy5 ( red dye) and cdna from abnormal cancer cells labelled with cy3 (green dye). Both of these cdnas are hybridized to a microarray chip. Which of the following is true if a scanned microarray chip identifies yellow at a specific transcript location: normal transcripts and abnormal transcripts are present at equal abundance. Expression vectors: for cloning/ genomic library production, do not need to express dna, lacz (multiple re sites), ampr, origin replication.
