Biology 1202B Lecture Notes - Lecture 20: Ecori, Restriction Enzyme, Guanine
24 Apr 2013
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Biggest issue with pcr is contamination as it only takes one cell to skew the results. Control is very important for any experiment. Positive control picking something that you know will give you the results you expect. Negative control expected to give you no results (ie. in pcr no amplification) Ladder, positive control and negative control are all important. If there is a band in the negative control, check if the pcr worked. If it amplified then it did not work. A recent paper reports that the presence of an autosomal recessive snp (guanine changed to adenine) in the hair texture gene correlates with curly hair. Looking at the sequence surrounding the genetic variation, you notice that this guanine nucleotide is in an ecori restriction enzyme sequence recognition site. In particular, if the guanine nucleotide is changed to an adenine nucleotide, this eliminates a restriction enzyme cut site.
