Biology 2290F/G Lecture Notes - Lecture 1: Calcium Chloride, Ampicillin, Plasmid
18 Apr 2017
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Lecture notes: coli jm101 (species and strain) Put it in calcium chloride, adding te buffer in one tube and the other one got pgreen. Control: te buffer - used to dissolve/resuspend dna. Experimental: pgreen - plasmid; already dissolved in te buffer. Confluent growth - there is so much growth you cannot see colonies. Ampicillin prevents proper cell wall formation and the structure that gets affected is the peptidoglycans (fibers) Ampicillin destroys the enzyme that crosslinks peptidoglycans. Negative control - hoping to see no growth. Cells can change physiologically (eg. putting it in ice) and result in growth. Only when there is a genetic modification. Maybe they became resistant or they already were resistant. Positive control - hoping to see lots of growth. Any plate that has growth can be called a positive control. High concentration of calcium chloride, ice (but that is ok since they"re good with ice) and heat (45 degrees)
