Biology 3660A/B Lecture Notes - Lecture 6: Trehalose, Protozoa
20 Jun 2015
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Anhydrobiosis life without water: ability dry out completely and rehydrate again, employed by small aquatic animals, protozoa, bacteria and plants (mostly fresh water, dries out faster, nematodes, tardegrades, rotifers, all are small, 5 6mm long, have little/no control over waterloss, are generally in habitats that are ephemerally wet (dry out a lot, all accumulate trehalose (2 glucose joined together) important, all have late embryogenesis abundant proteins (lea proteins) Midge larva: ephemeral pools in w. africa, largest known anhydrobionts, requires slow drying, accumulated large amounts of trehalose, has trehalose transporters for getting it form hemoceol to cells, hsps, leas, etc , read paper on owl, vitrification! Experimental design fast dehydration 4. 2 trehalose, 3% water content, 0% survive slow dehydration 277 mg, 3% wc, 91% survive: only difference in trehalose content assumed that it is the main factor, where is the trehalose, did it per mg of protein, standardized the trehalose to protein amount, evenly distributed in animal.
