Biology 2290F/G Lecture Notes - Lecture 5: Ferrari 250, Acid Dissociation Constant, Buffer Solution

• School: Western University
• Department: Biology
• Course: Biology 2290F/G
• Professor: Ray Zabulionis

Document Summary

Use n = c x v and a = ec. R = c/(cid:373)i(cid:374) x v = a/(cid:2923)i(cid:2924) x v. **= (cid:373)ol/l (cid:862)dcpip(cid:863) redu(cid:272)ed/(cid:373)i(cid:374)** **v i(cid:374) l (cid:373)ol (cid:862)dcpip(cid:863) redu(cid:272)ed/(cid:373)i(cid:374)** Resolution (d) is the smallest distance by which two objects must be separated in order to allow them to be imaged as two separate objects. Abbe"s equatio(cid:374): provides the theoretical limit of resolution in an optical system d = 0. 612 / (sin) **sin is the numerical aperture of the lens & = refractive index** air (air = 1) e. g. immersion oil; angle should be close to 90 ) Maximum resolution with a light microscope (small d): should (cid:271)e (cid:373)i(cid:374)i(cid:373)ized, a(cid:374)d the numerical aperture should be maximized (substance with higher refractive index than. Haemacytometer: type of microscope slide: precisely etched lines that intersect to form squares of known area, a known volume of fluid is enclosed above each square (proper cover, steps: